Figure 4.
Detection of IFN-γ and granzyme B protein secretion by ELISA. The capacity of T cells to secrete cytokines and effector molecules was assessed by determining the secretion levels of (A) the Th type 1 (Th1) cytokine IFN-γ and (B) granzyme B by ELISA. After stimulation with HSP70, CMVpp65495-503 peptide, and HSP70/CMV-PC the T-cell culture supernatants were harvested on days 7, 14, and 21 and used for analysis. The results of independent experiments are expressed as means (weak: n = 5, medium: n = 5, strong: n = 6) are expressed as mean ± SD. Asterisks shown in the Figure indicate only statistically significant differences between levels in CMVpp65495-503 peptide- and HSP70/CMV-PC-stimulated cells (* p < 0.05, ** p < 0.01, *** p < 0.001).