Table 1.
Classification of the three responder groups (weak, medium, and strong) after stimulation with CMVpp65495-503 peptide.
| day 0 | day 7 | ||||
|---|---|---|---|---|---|
| donor | percentage of pentamer+CD8+ T cells | absolute number of pentamer+CD8+ T cells [x106] | percentage of pentamer+CD8+ T cells | absolute number of pentamer+CD8+ T cells [x106] | responder classification |
| 1 | 0.40 | 0.04 | 0.30 | 0.02 | weak |
| 2 | 0.39 | 0.04 | 0.19 | 0.02 | weak |
| 3 | 0.39 | 0.04 | 0.34 | 0.06 | weak |
| 4 | 0.37 | 0.04 | 0.50 | 0.07 | weak |
| 5 | 0.40 | 0.04 | 0.14 | 0.02 | weak |
| 6 | 0.30 | 0.03 | 4.50 | 0.56 | medium |
| 7 | 0.53 | 0.05 | 5.19 | 0.36 | medium |
| 8 | 2.17 | 0.22 | 2.75 | 0.17 | medium |
| 9 | 0.79 | 0.08 | 2.89 | 0.38 | medium |
| 10 | 0.30 | 0.03 | 1.11 | 0.19 | medium |
| 11 | 0.65 | 0.07 | 37.76 | 4.34 | strong |
| 12 | 5.64 | 0.56 | 13.54 | 1.15 | strong |
| 13 | 0.56 | 0.06 | 21.00 | 1.26 | strong |
| 14 | 1.40 | 0.14 | 16.67 | 2.17 | strong |
| 15 | 0.70 | 0.07 | 16.80 | 1.60 | strong |
| 16 | 1.70 | 0.17 | 19.50 | 1.37 | strong |
The data sets from 16 healthy HLA-A*02:01/CMV-seropositive donors were divided into 3 responder groups: weak (n = 5), medium (n = 5), and strong (n = 6), respectively, according to the obtained frequency of generated A2/CMV-pentamer-positive CD8+ T cells after 7 days of stimulation with the CMVpp65495-503 peptide (weak responders < 1% A2/CMV-pentamer-positive CD8+ T cells, medium responders 1-10% A2/CMV-pentamer-positive CD8+ T cells, strong responders > 10% A2/CMV-pentamer-positive CD8+ T cells).