Figure 5.

Resveratrol (RSV) enhances basal and ionizing radiation (IR)-induced AMPK phosphorylation. A. Effects of IR alone: PC3 cells were treated with 0 or 8 Gy IR and lysed at the indicated time points after IR exposure. The adjacent panel shows PC3 cells treated with 8 Gy IR, which were lysed 1 hour after IR treatment. Samples were subjected to immunoblot analysis using the indicated antibodies. B. RSV enhances basal AMPK phosphorylation: PC3 and 22RV-1 cells were treated with the indicated concentrations of RSV for 1 hour followed by lysis and immunoblotting analysis. C. RSV enhances IR-induced AMPK phosphorylation. PC3 and 22RV-1 cells were pre-treated with the indicated concentrations of RSV 1 hour prior to 8 Gy IR treatment. Cells were lysed 1 hour after IR exposure and subjected to immunoblotting using indicated antibodies. Shown are representative immunoblots of at least 3-4 independent experiments. D. Densitometry-based quantitation. Density values of P-AMPK were normalized to T-AMPK levels and presented as fold change compared to untreated control cells (mean ± SE) from 2-3 independent experiments in C. is shown. * p < 0.05 compared to cells treated with IR alone.