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Primers used for cloning and sequencing
| primers | sequences | fragments (bp) |
|---|---|---|
| EV71-VP1-1F | 5'-TGAAGTTRTGYAAGGATGC-3' | |
| EV71-VP1-1R | 5'-CCACTCTAAAATTRCCCAC-3' | 993 |
| EV71-VP4-1F | 5'-CTACTTTGGGTGTCCGTGTT-3' | |
| EV71-VP4-1R | 5'-GGGAACTTCCAGTACCATCC-3' | 655 |
| CA16-VP1-1F | 5'-ACTATGCAAGGACACWGAG -3' | |
| CA16-VP1-1R | 5'- CAGTGGTGGAAGAGACTAAA-3' | 1076 |
| CA16-VP4-1F | 5'- GGCTGCTTATGGTGACAA-3' | |
| CA16-VP4-1R | 5'- CATGGGAGCTATGGTGAC-3' | 1090 |
F referred as forward primer and R referred as reverse primer.
