Table 5.
Primers designed for the expression of VP1s and VP4s
| primers | sequences | endonuclease | fragments (bp) |
|---|---|---|---|
| EV71-VP1-2F | 5'- ACGGATCCGGAGATAGGGTGGCAGAT -3' | BamHⅠ | |
| EV71-VP1-2R | 5'- CCTCTCGAGTTAAAGAGTGGTGATCGCTGTG -3' | XhoⅠ | 911 |
| EV71-VP4-2F | 5'-CCGAATTCATGGGCTCGCAAGTG-3' | EcoRⅠ | |
| EV71VP4-2R | 5'-TCTCTCGAGTTACTTCAGCGGCGCTGC-3' | XhoⅠ | 227 |
| CA16-VP1-2F | 5'- GAAGATCTGGGGGATCCTATCGCAGAC-3' | BamHⅠ | |
| CA16-VP1-2R | 5'- CCGCTCGAGTTACAGTGTTGTTATCTTG-3' | XhoⅠ | 912 |
| CA16-VP4-2R | 5' -CGCCGAATTCATGGGGTCACAAGT -3' | EcoRⅠ | |
| CA16-VP4-2F | 5'- TATCTCGAGTTACTTGAGCGGCGGG -3' | XhoⅠ | 229 |
F referred as forward primer and R referred as reverse primer.