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. 2011 Oct 26;11:145. doi: 10.1186/1471-2229-11-145

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Copyright ©2011 Hunt et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Identification of the authentic tag corresponding to its Glyma model. (A) Clark standard (CS) Glyma04g35130 transcript sequence. Underlined sequences represent DpnII restriction sites. DGE0000012, indicated in red is an authentic tag because it is adjacent to the last DpnII site in the 3'UTR sequence of this gene. Other non-authentic site tags on either the sense or antisense strand are also shown: DGE0002838 (yellow) and DGE0022468 (green) originated from restriction fragments which are not washed after digestion of cDNA with DpnII; DGE0008244 (ferozi) and DGE0033570 (grey) originated due to inefficient restriction of cDNA by DpnII. (B) Five DGE tags match Glyma04g35130 sequence. Their respective sequences and counts in CS and the glabrous-mutant (CG) are indicated.