Table 1.
Oligonucleotides developed for Eimeria species-specific LAMP PCR.
| Species | Target | Acc. no. | Primer ID | Sequence (5' - 3') |
|---|---|---|---|---|
| E. acervulina | Ac-AD18-953a | AY571534 | Eac_F3 | CCTAACATTTCGCTTCACGGAC |
| Eac_B3 | ATGAGCAAGTGGAACACCTTG | |||
| Eac_FIP | AGAGCACAGTGGCAGTGC-AGCAGACAGCATGGCTTACCT | |||
| Eac_BIP | GAAGACCCTCTGAAGAACGGA-CCTTCTCACCGCTTACCGG | |||
| Eac_LB | TAAGGTTACACCCGTGGAGG | |||
| Eac_LF | GCCATGCACAAAGCGACTT | |||
| E. brunetti | Br-J18-626a | AY571556 | Ebr_F3 | GGCCATCAAGTTCCATGAGC |
| Ebr_B3 | TCAACCTCCTGAGTGTGGTT | |||
| Ebr_FIP | GAAAATGCCTTCGTAGCTGCT-GCTGGGTACGGAGCGTCTT | |||
| Ebr_BIP | TACTTCCTAGGATCCATCCTCGC-AGTTTCGCTGCCGCCTC | |||
| Ebr_LB | GAAACGCTCGAACATGGC | |||
| Ebr_LF | CTTCTCCACAGACCCAGAGGT | |||
| E. maxima | EmMIC1 | M99058 | Ema_F3 | ACTACGGAAAAGTGCGTAGCT |
| Ema_B3 | CCTTCCTCCCTTCTGAAAACTG | |||
| Ema_FIP | GAGTCACTGCTGATGTACCAAAAG-GAACTATGCCGCTTTCCCCTG | |||
| Ema_BIP | AGAATGCGGATTTGTTAGCAGC-AGCAAGTCCAAGGTGTGTGTA | |||
| Ema_LB | CAAGCCTACGCGGACATC | |||
| Ema_LF | TTATGCAGCTGGGTCAACG | |||
| E. mitis | Mt-A09-716a | AY571506 | Emi_F3 | ACGATAGCCAAGACACGTAAGG |
| Emi_B3 | CCCCGTGATAAGAGTAGGAACA | |||
| Emi_FIP | CGCGGGTCGTGAGATTTAAATTAT-GGAAGATCAGGACGGGCACT | |||
| Emi_BIP | GTTTCAGTTGATGAACAAGCGAGA-TGCGCCTCTAGAATCAAGACG | |||
| Emi_LB | TCCATGCATCCCCTTGTT | |||
| Emi_LF | CGTGGGCACAGATTGATTC | |||
| E. necatrix | Nc-AD10-702a | AY571565 | Ene_F3 | TGGCTTTCCCGCGTACC |
| Ene_B3 | CGGCCCAACACAAAGACTG | |||
| Ene_FIP | CGCTTGAGTTTTAAGCTATGCACA-GACCCAAGCAGCTCACCAA | |||
| Ene_BIP | CGCCATGCCATTCAATGAACG-GAGGCATACCGGCGTTGTC | |||
| Ene_LB | GTCTGTAACTTGGGACGTTGT | |||
| Ene_LF | GAACAGCCGGAGCCTCTC | |||
| E. praecox | Pr-A09-1108a | AY571603 | Epr_F3 | GCCCTTGTATGTTGCTGTTTCT |
| Epr_B3 | GCGCACGAATCTGAATCACAC | |||
| Epr_FIP | ATCTCCTCAAAGACTTTCGCGTA-GCGCTTGGCTATATCCATAGG | |||
| Epr_BIP | GCTCTCGTGGCATACTTGC-GCCAGGAGCCACTGATTGT | |||
| Epr_LB | GAATAGCATTGCCAGGTGG | |||
| Epr_LF | GTCCACTGTCATTAATATTGCTGC | |||
| E. tenella | Tn-E03-1161a | AY571629 | Ete_F3 | GCTTGTGAAGGTCAGCGTG |
| Ete_B3 | GCTGAGTCCATACGTACTTCCT | |||
| Ete_FIP | GCCACTGCTATGGAAAGTCACAC-CATAACTGGCATGCAGGGGT | |||
| Ete_BIP | GTTTGGCCCGAAAGTTGTGAAGA-CGTCAGAAATTGCTGCCCAAT | |||
| Ete_LB | CGCATGTGCAGTTGAAGACA | |||
| Ete_LF | CCAAATGTATCTGCTAGTTATATTAACAAG |
aEimeria species-specific SCAR markers [30].