. Author manuscript; available in PMC: 2012 Nov 15.

Published in final edited form as: Biochemistry. 2011 Oct 19;50(45):9845–9856. doi: 10.1021/bi2011306

Table 2.

Equilibrium and Kinetic Parameters for PH domains Binding to Plasma Membrane Mimics Containing PIP Lipids

PH Domain	K_D(IP₆) (μM)	Plasma Membrane Mimic^a	Equilibrium		Kinetics
PH Domain	K_D(IP₆) (μM)	Plasma Membrane Mimic^a	K_I(IP₆) (mM)	K_D(PIP_n) (nM)	k_on (×10⁶ M⁻¹ s⁻¹)	k_off (s⁻¹)	K_D (k_off/k_on) (nM)
WT	20 ± 3	PM(+)PI(3,4,5)P₃	5 ± 2	14 ± 8	14 ± 1	0.79 ± 0.01	55 ± 1
		PM(+)PI(4,5)P₂	0.04 ± 0.01	3000 ± 2000	ND^b	ND^b	—
E345K	4.1 ± 0.1	PM(+)PI(3,4,5)P₃	7 ± 2	1.7 ± 0.1	20 ± 1	0.042 ± 0.007	2.1 ± 0.3
		PM(+)PI(4,5)P₂	0.026 ± 0.005	400 ± 50	53 ± 1	23 ± 1	430 ± 20
E345K/I307A	8 ± 2	PM(+)PI(3,4,5)P₃	14 ± 4	1.7 ± 0.7	35 ± 1	0.16 ± 0.01	4.6 ± 0.1
		PM(+)PI(4,5)P₂	0.035 ± 0.005	800 ± 100	32 ± 1	18 ± 1	550 ± 20
PLCδ1	4 ± 1	PM(+)PI(3,4,5)P₃	ND^b	> 10⁴^c	ND^b	ND^b	—
		PM(+)PI(4,5)P₂	0.07 ± 0.01	150 ± 20	180 ± 10	15 ± 1	81 ± 5

PIP lipid level is 2 mole percent (Table 1).

Not determined due to insufficient affinity.

Lower limit determined as previously described (2).