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Effect of cell density on PGK1 release. HeLa cells were plated at the indicated cell densities in MEM/10% NBS. After 24 h, the cells were washed with serum-free MEM and then incubated with the same media for 2 h. The amount of PGK1 released into the media was determined using an ELISA. Error bars represent the s.d. of the mean (n = 4). AU, arbitrary units.
