Figure 5. Activity assay of bat Leptin.

Relative effect of bat Leptin on viability (MTT) (A) and cytotoxicity of 3T3-L1 adipocytes (LDH) (B). 3T3-L1 cells were incubated with bat GST-Leptin (10⁻⁶ M) at 24h or 48h. Cell viability and cytotoxicity were tested by the MTT and LDH assay with four to six replicates for each treatment and experiments were repeated three times. Data are means ± SD. Asterisk indicates significant difference (* P<0.05; t-test).