Table 3. Antibodies used in immunocytochemistry and Western blotting.

Primary Antibodies	Manufacturer	Immunocytochemistry (Dilution)	Western blotting (Dilution)	Characterization
Anti-cow ubiquitin (rabbit)	DAKO	1∶200	1∶200	Stains only expected band of ∼10 kDa on Western blot of Monodelphis spinal cord tissue which was confirmed by mass spectrometry (Figure 4)
Anti-human pan-14-3-3 (rabbit)	Biomol International	-	1∶500	Stains two expected bands ∼30 kDa of Monodelphis spinal cord tissue which was confirmed by mass spectrometry (Figure 4)
Anti-N-terminus 14-3-3 epsilon (rabbit)	Biomol International	-	1∶2000	Stains one expected band ∼30 kDa of Monodelphis spinal cord tissue which was confirmed by mass spectrometry (Figure 4C)