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. 2011 Nov 16;6(11):e27586. doi: 10.1371/journal.pone.0027586

Figure 5. Real-time RT-PCR of adhesion molecules E-selectin, VCAM-1, and ICAM-1.

Figure 5

HUVECs transduced with the control vector, GPR4 or GPR4 R115A mutant were treated with EGM-2/HEM media at pH 8.4, 7.4, or 6.4 for 5 h. Total RNAs were isolated and reverse transcribed. Real-time RT-PCR quantification of mRNA levels of E-selectin (A), VCAM-1 (B), and ICAM-1 (C) was performed in duplicate. Values were normalized to the housekeeping gene GAPDH. The expression level of the target gene in HUVEC/Vector cells at pH 8.4 was set as 1. The results are representative of at least two independent experiments. Error bars are the mean ± SEM.