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. 2011 Nov 16;6(11):e27586. doi: 10.1371/journal.pone.0027586

Figure 7. Blockade of E-selectin, VCAM-1 or ICAM-1 reduces the adhesion of HUVECs with U937 monocytic cells.

Figure 7

HUVECs stably overexpressing GPR4 were treated with different pHs as indicated for 5 to 15 h, followed by 30 min incubation with pH 7.4 medium containing 5 µg/ml IgG1 control antibody, anti-E-selectin, anti-VCAM-1, or anti-ICAM-1 antibody. The cell adhesion assay was then performed as described under “Materials and Methods”. **, P<0.01; ***, P<0.001; compared with the IgG1 control group at pH 6.4. The results are representative of two independent experiments. Error bars are the mean ± SEM.