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. 2011 Nov 16;6(11):e27385. doi: 10.1371/journal.pone.0027385

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Allantois explants were cultured in the presence of inhibitor or its vehicle, fixed, stained and microscopic photographs taken as described. (A) Representative images of a control (top) and a Y-27632 treated (bottom) allantois explant (left), their skeletonised images (middle panels) and a representative, enlarged part of the skeletonised images shown in the middle panels. The vessels outlines are represented in yellow, the skeleton in red and the branching points in blue. (B) Graphical representations of the analysis with AngioTool performed on seven control and ten Y-27632 treated explants. Statistical analysis was by T-test (Mann Whitney) *, p<0.05; **, p<0.01.