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. 2011 Nov 16;6(11):e27385. doi: 10.1371/journal.pone.0027385

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The control allantois explants for the experiment shown in Fig. 5 were overlaid according to the pre-set parameters (skeletons not optimised) or after careful optimisation. This lead to significantly different branching indices (B; p<0.001, Mann Whitney T-test). (A) shows an enlarged area of a stained explant; the same area is shown after skeletonisation using the pre-set parameters (C) or after optimisation (C'. A few examples of missed vessels and junctions in the non-optimised skeleton are highlighted by ringed areas and arrowheads.