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A) Antisens, RNA-in situ demonstrates AQP8 in normal colonic columnar epithelium from one patient. B) Sens, RNA-in situ demonstrate no signal. C) Antisens, HNPCC carcinoma demonstrates that there is no AQP8 expression in the tumor. Epithelial cells of the crypt formation on all photos give an autoflorescence seen as white borders in the pictures, the probe hybridization is seen as small white dots.
