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. 2011 Nov 16;6(11):e27736. doi: 10.1371/journal.pone.0027736

Figure 1. Northern blot analyses of Actin, ATPase, Hsp70 and CLIC mRNAs in B. cockerelli and D. citri.

Figure 1

Total RNAs (1.5 µg) from B. cockerelli (BC) and D. citri (DC) were separated by denaturing agarose gel electrophoresis, transferred to nylon membranes and hybridized with 32P-UTP-labeled probes prepared using corresponding B. cockerelli sequences. 0.5–10 Kb RNA ladder (Invitrogen) was used on the same gel and the sizes are indicated. Labels A – D above each panel indicate the specific probe used for the corresponding blot. The exposure times for BC-Actin, BC-ATPase, BC-Hsp70 and BC-CLIC were 15 h, 2 h, 38 h and 38 h, respectively.