. 2011 Nov 16;6(11):e27736. doi: 10.1371/journal.pone.0027736

Table 2. Quantitative real-time PCR detection for endogenous B. cockerelli mRNAs after feeding of dsRNAs or siRNAs.

RNA type¹	RNA sample from²	NO. of sample³	Sequence name⁴	Means ± SE in GFP sample⁵	Means ± SE in Test sample⁵	P Value⁶
dsRNA	WS	4	BC-Actin	1±0.25	1.21±0.30	0.4115
dsRNA	WS	6	BC-Actin	1±0.42	0.88±0.31	0.5776
dsRNA	Gut	2⁺	BC-Actin	1±0.04	0.29±0.02	0.0021**
dsRNA	Gut	2⁺	BC-Actin	1±0.18	0.36±0.03	0.0095**
dsRNA	WS	7	BC-ATPase	1±0.30	0.35±0.15	0.0002**
dsRNA	WS	5	BC-ATPase	1±0.27	0.63±0.18	0.0343*
dsRNA	WS	7	BC-ATPase	1±0.16	0.51±0.14	0.0002**
dsRNA	WS	4	BC-ATPase	1±0.25	0.42±0.39	0.0450*
siRNA	WS	8	BC-ATPase	1±0.27	0.56±0.20	0.0025**
siRNA	WS	6	BC-ATPase	1±0.37	0.39±0.09	0.0029**
dsRNA	WS	7	BC-Hsp70	1±0.25	0.34±0.14	0.0001**
dsRNA	WS	5	BC-Hsp70	1±0.26	0.63±0.12	0.0213*
dsRNA	WS	5	BC-CLIC	1±0.18	0.49±0.23	0.0049**
dsRNA	WS	5	BC-CLIC	1±0.07	0.82±0.06	0.0027**

1. Psyllids were fed with dsRNA or siRNAs as indicated.

2. Total RNA was isolated from individual psyllid or gut samples from a pool of 30 insects. WS, whole insect.

3. The number of psyllids used for qRT-PCR analysis in one experiment. For each experiment, the same numbers of GFP samples were used as controls for test sample. ⁺For gut sample, a pool of 30 psyllids was used for every treatment.

4. The name of sequences used for dsRNA feeding and mRNA detection by qRT-PCR.

5. The mRNA abundance of specific genes after introduction of corresponding dsRNA or SiRNA was shown as test sample, and the average value of the control GFP group was designated as 1. Expression of each mRNA was normalized to the level of rRNA in the same sample.

6. Difference between GFP group and test group was calculated and shown as P value using the Bonferroni (Dunn) t-test. Single asterisk indicates p<0.05 and double asterisk indicates p<0.01.