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. 2011 Nov 16;6(11):e27815. doi: 10.1371/journal.pone.0027815

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Takeda et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) SPR sensorgrams of the interactions between GST-Vpr and the full-length Impα isoform, NPI-1_full, immobilized to the CM sensor chip. Sensor curves of the interactions between Vpr at various concentrations (0 to 40 µM) and NPI-1_full are shown. (B) The analysis curves used to obtain the dissociation constants (K_D) for the interactions between Vpr and the Impα isoforms, Rch1_full, Qip1_full, NPI-1_full, Rch1_404-529, Qip1_392-537 and NPI1_404-541 using the steady state binding model equation (see Material and Methods).