Table 1. Dissociation equilibrium constants determined using BIAcore.
Analite | Ligand | KD (M) | Ligand | KD (M) |
Vpr | Rch1full | 8.9×10−6 | Rch1404–529 | 4.3×10−6 |
Qip1full | 6.8×10−6 | Qip1392–537 | 6.5×10−6 | |
NPI-1full | 7.4×10−6 | NPI-1404–541 | 6.7×10−6 |
Dissociation equilibrium constants determined using BIAcore. KD of Vpr was determined by using the Vpr sensorgrams obtained by subtracting GST sensorgrams from GST-Vpr sensorgrams at the same concentrations. Full: full-length.