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. 2011 Nov 16;6(11):e27758. doi: 10.1371/journal.pone.0027758

Figure 7. Primary DNA sequences of the B95-8 and the Akata FR.

Figure 7

(A) The previously reported B95-8 FR sequence (AJ278309) [5] aligned along palindromic cores of each repeat unit. Nucleotides constituting 12 bp palindromic core of each repeat unit are in bold letters, and those that are mismatched to the consensus motif (TAGCATATGCTA) of the palindromic core are highlighted in yellow. Nucleotides G, A, T, and C at position −7 and +7 are labeled with different colors to emphasize the orientation of each repeat unit. Nucleotides in concordance with the GenBank sequence of the B95-8 strain EBV (V01555) is highlighted in gray, while the 252-bp sequence missing in V01555 is surrounded by dotted lines. Underlined nucleotides represent 128-bp nearly perfect palindrome. Nucleotide numbers in parentheses are as in Fig. 1B. (B) Two Akata-derived BAC clones that were subjected to DNA sequencing of their FR regions were analyzed by Southern blot as in Fig. 3D. (C) B95-8 genomic DNA and Virion DNA of the Akata strain EBV were subjected to PCR analysis, in the absence or the presence of betaine, using a set of primers flanking the FR region. (D) Primary DNA sequences of Akata FR23 (AB644408) and FR18 (AB644409). Bold letters, colored letters, and yellow highlights are as in (A). Identical nucleotides between FR23 and FR18 are highlighted in gray, while discrepancies are surrounded by dotted lines.