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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Dev Biol. 2011 Jun 25;357(2):283–294. doi: 10.1016/j.ydbio.2011.06.025

Figure 7. Hem and Abl independently regulate WAVE and the migration of RP2.

Figure 7

Panel A: Western analysis of embryo extracts for WAVE. Panels A and B: Western analysis of extracts for WAVE from Abl zygotic and maternal and zygotic mutant embryos from 6-10- hrs and 12-16-hrs of age. Panel C: Abl maternal and zygotic mutant embryos are of small size compared to wild type. Panel D: Gain-of-function for Abl (UAS-Abl X sca-GAL4) only marginally reduces the levels of WAVE between 5-9-hrs of age (during the time of RP2 migration). Panel E: The levels of WAVE in wild type (lane 1), Hem single mutant (lane 2), Hem, Abl (zygotic) double mutant (lane 3) and Abl zygotic mutant (lane 4). Panel F: Hem, Abl zygotic double mutant embryo stained with Eve. Panel G: Table showing the penetrance of the RP2 mis-migration defect. The intensity of the signals for WAVE, normalized against the loading control Tubulin, is given below each Western.