Table 3.
List of the primers used for real-time qPCR
| Genes | Accession N° | Primers sequence (5'-3') | |
|---|---|---|---|
| Housekeeping gene | |||
| gapdh | Glyceraldehyde 3-phosphate dehydrogenase | DQ403055 | F: CAATGACCCCTTCATTGACC R: GACAAGCTTCCCGTTCTCAG |
| Regulation of cytoskeleton | |||
| tubb2b | β-Tubulin | NM_023716 | F: GCAACATGAATGACCTGGTG R: ACCAGAGACCCAGCACAAAC |
| thy1 | Thy1 Antigen | NM_009382.3 | F: AAGGCCTCTGCCTGTAGTGA R: GAAGAGGCAGGTTGCAAGAC |
| actin | β-Actin | NM_007393 | F: CACCACCACAGCCGAGAG R: CCAGGGAGGAAGAGGATGC |
| col1a1 | Collagen α1 | NM_000088 | F: GGGTCATTTCCACATGCTTT R: TCCGGGTTTCAGAGTACCAC |
| thbs1 | Thrombospondin 1 | NM_011580 | F: CCAAAGCCTGCAAGAAAGAC R: CCTGCTTGTTGCAAACTTGA |
| plekha5 | Plekstrin homology A5 | NM_019012 | F: GTGCATCTGCCTGAAGACAA R: TGGGAACCTTTAACGACTGG |
| kif23 | Kinesin 23 | NM_024245.4 | F: CCTGAGCTTTCCTGACCAAG R: AGTTCCTTCTGGGTGGTGTG |
| has2 | Hyaluronan synthase 2 | NM_008216 | F: CGGAGGACGAGTCTATGAGC R: TTTTCCGGTGTTCCAAAAAG |
| flrt2 | Fibronectin leucine rich 2 | NM_201518 | F: ACCGCACTGTGGAAGATACC R: GCAAGACAACGAGCACAAAA |
| enah | Enabled homolog | NM_008680 | F: GCCTATGCTTCAGCACTTCC R: GGGCGATTGTCTTCTGACAT |
| dclk1 | Doublecortin like 1 | NM_019978 | F: AGCCTCCACCAGCTCAGTTA R: CCATACACATCGCTCCATTG |
| ctnnbip1 | Catenin β interacting protein 1 | NM_020248 | F: TTGGCTGCAGAAAGAAACCT R: CCAGCCAATCACAACCTTTT |
| crip1 | Cystein rich protein | NM_007763 | F: AGTCCAGAGCCTGCAACCTA R: GGAGTAGCAGGGATGATTGC |
| coro1c | Coronin | NM_014325 | F: GCAGAAGAGTGGTTCGAAGG R: TGATCAGGTCGCACTTCTTG |
| cdh3 | Cadherin 3 | NM_007665 | F: CACACGACCTCATGTTCACC R: CTGTACCTCATGGCCCACTT |
| calml3 | Calmodulin like 3 | NM_027416.3 | F: ATCGACAAGGATGGAAACG R: ATCTACCTCCTCGTCGCTCA |
| cttnbp2 | Cortactin binding protein 2 | NM_030249 | F: GACAAAGAAGGCTGGACTGC R: CTCACCCACGGAAATCCTTA |
| lrrc8a | Leucine rich repeat 8A | NM_177725 | F: AGAGCCCACTTACCCCAACT R: GTGTGCAGAAGCACGAGGTA |
| snx6 | Sorting nexin 6 | NM_021249 | F: CCAAGACCTGATTTTGATGCTTC R: CATGCATCGCAACTGTCTTC |
| nrp2 | Neuropilin 2 | NM_0010774 | F: ATAAGCACTGATGTCCCACTG R: GAGTTGCTCCAATCTCCTTCA |
| nid2 | Nidogen 2 | NM_008695 | F: CTCATTCAGTTGTGCCTGC R: ATAGCTGCCTCATGACATCG |
| Regulation of apoptosis | |||
| pi3kr1 | phosphatidylinositol 3-kinase (p85a) | NM_001077495 | F: CGAGCCCGACCGGAGGTGAA R: CGCACACTGCCGTCCGAGTT |
| bcl-2 | B-cell lymphoma 2 | AJ582074 | F: CGCAGAGATGTCCAGTCAGC R: CGAACTCAAAGAAGACCACAA |
| c-myc | cellular myelocytomatosis oncogene | AJ582076 | F: GACCCTGATTCGGACCTCTT R: CGACTCCACAGCCTTCTCTC |
| p53 | tumor supressor p53 | U07182 | F: ATGACGGAAGTTGTAAGAC R: TCGGATAAGATGCTGAGG |
| PPARs genes | |||
| ppar α | peroxisome proliferator activated receptor alpha | AY170844 | F: GTTTCTTTCGGCGAACTATT R: ACACGTGAGAATCTCTGCTT |
| ppar β/δ | peroxisome proliferator activated receptor beta/delta | AF486582 | F: TGCAAGATCCAGAAGAAGAA R: GTAGATGTGCTTGGAGAAGG |
| ppar γ | peroxisome proliferator activated receptor gamma | AB525757 | F: GGACCTCTCTATGATGGATG R: GGATGCAGGTTCTACTTTGA |
| CYP4 family genes | |||
| cyp4a17 | Cytochrome P450 4A17 | AJ555628 | F: ACCAGATGCCCTACACTACC R: GTGCGTAAATGGAGAGTACA |
| cyp4a18 | Cytochrome P450 4A18 | AJ555629 | F: ACCAGATGCCCTACACTACC R: GGCCATAAATGGAGATTGCA |
| cyp4a19 | Cytochrome P450 4A19 | AJ555630 | F: ACCAGATGCCCTACACTACC R: GTGCATAAATGGAGAGTGTG |