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. Author manuscript; available in PMC: 2012 Oct 25.
Published in final edited form as: Curr Biol. 2011 Oct 13;21(20):1695–1703. doi: 10.1016/j.cub.2011.08.056

Figure 2. Dig1 and Ste12 are bound to centromeric chromatin.

Figure 2

(A) Dig1-Ste12, Cdc14-Net1 and Sum1-Hst1 are bound to centromeric chromatin in vitro. In vitro kinetochore assembly system was executed as described in Figure 1. The protein extracts derived from the tagged strains were as follows: Dig1-myc Ste12-myc (Y2052); Cdc14-myc Net1-HA (Y2050); and Sum1-myc Hst1-myc (Y2051). (B) Anti-myc chromatin immunoprecipitation (ChIP) assays were performed from log-phase cells. Total lysate (T) and coimmunoprecipitated DNA (IP) were analyzed by PCR with primers specific to centromeric regions of chromosomes III, XI, and XVI and to a noncentromeric region (PGK1). The yeast strains used were as follows: untagged (YPH499), Cbf1-myc (Y2053), Dig1-myc (Y2054), and Ste12-myc (Y2055).