Figure 3.
Effect of gastrin on Panc-1 cell adhesion. Cells were added to fibronectin-coated (A) or non-coated (BSA alone) (B) 96-wells for 2 h in the presence or absence of gastrin. Adherent cells were fixed and stained with crystal violet as described in Materials and Methods. After solubilisation, absorbance was measured at 570 nm. When indicated, Panc-1 cells were pre-incubated, with a blocking αV mAb for 30 min prior to gastrin treatment for 2 h. Quantifications of three experiments are presented as mean ± SE. Significance was accepted at P ≤ 0.05. bP < 0.01. BSA: Bovine serum albumin.