Figure 4.

Failure of CFTR activation in trp4 deficient MAEC A. Current traces from a wild type MAEC obtained from steps protocols (voltage steps from +80 to -80 mV, decrement 40 mV, holding potential was V_H = 0 mV). B. Same protocol as in panel A, but after application of the phosphorylating cocktail C. Same protocol as in A, but for a trp4 deficient cell. D. Same protocol as in C for the trp4 -/- cell after stimulation with the phosphorylating cocktail. E. Pooled current densities at +50 mV (obtained from voltage ramps) in wild type and trp4 deficient MAEC under resting conditions (cont) and after stimulation with the phosphorylating cocktail (cAMP). Note the lack of current activation by the phosphorylating cocktail in the trp4 deficient MAEC. Amplitude of the cAMP activated current in wild type (+/+) and trp4 deficient (-/-) endothelial cells, i.e. the difference between the current amplitudes in stimulated and unstimulated cells in panel E.