Effects of chloral hydrate-induced anesthesia on AMPA receptor subunit expression in surface (S) and intracellular (I) pools of mouse cortical neurons. (A, C, and E) Effects of anesthesia on GluR1 (A), GluR2 (C), and GluR3 (E) expression in the two pools. (B, D, and F) Effects of anesthesia on the ratio of surface to intracellular expression of GluR1 (B), GluR2 (D), and GluR3 (F). Anesthesia was induced by chloral hydrate (CH) at 400–500 mg/kg (i.p.). Mice were sacrificed 15 min after drug injection. Representative immunoblots are shown left to the quantified data (A, C, and E). The data are expressed in terms of means ± SEM (n = 6 per group). *: P < 0.05 versus vehicle.