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SDS-PAGE and MALDI-TOF MS analysis of GlcNAc-Fc and transglycosylation product Fc-1. A, SDS-PAGE: Lane 1, HM-Fc; lane 2, GlcNAc-Fc; lane 3, transglycosylation product Fc-1; lane 4, PNGase F-treatment of Fc-1; B, MALDI-TOF MS of GlcNAc-Fc; C, MALDI-TOF MS of product Fc-1; D, MALDI-TOF MS of N-glycans released from Fc-1.
