Fig. 1.

Generation and characterization of HER2-Fc and Ctrl-Fc proteins. a Diagrams illustrating the cDNA structures of the two chimeric cDNAs. The coded amino acid sequence of the HER2 region used is shown (modified amino acids are underlined) (LS, leader sequence; HER2 indicates the amino acid stretch 364–391). b Western blot analysis of HER2-Fc and Ctrl-Fc. Proteins (1 μg/sample) were fractionated in nonreducing and reducing conditions (±β-mercaptoethanol) in SDS-PAGE 4–12% gradient gel and blotted onto nitrocellulose. Western blotting was performed using peroxidase-conjugated MoAb anti-Fcγ revealed by chemiluminescence