Fig. 2.

HER2-Fc cDNA vaccination and antitumor response elicited in BALB/c mice. Groups of mice were vaccinated twice at 2 weeks interval with empty pcDNA3.1 (n = 5; gray line), Ctrl-Fc (n = 10; dotted black line), and HER2-Fc (n = 11; black line) and challenged 1 week after the second vaccination with a lethal dose of D2F2/E2 cells transfected with the human HER2. a The percentage of tumor-free mice in function of time is represented. b Ability of vaccination to delay tumor growth. Each line refers to an individual tumor (pcDNA3.1, n = 5; Ctrl-Fc, n = 5; HER2-Fc, n = 5)