Skip to main content
. Author manuscript; available in PMC: 2012 Nov 23.
Published in final edited form as: J Am Chem Soc. 2011 Oct 31;133(46):18966–18974. doi: 10.1021/ja2082813

Fig. 3. Ionic strength dependence of CaM-Ca2+ PREs.

Fig. 3

Experimental PREs (circles, error bars = 1 s.d.) were measured for CaM-4Ca2+ with the nitroxide spin label at (A) S17C and (B) A128C at various ionic strengths. Intradomain PREs are shown in the left panels and interdomain PREs in the right panels. The data are presented as scatter plots with the PRE value at 0 mM KCl shown on the x-axis and the PRE value at 100 mM (red) and 200 mM (blue) shown on the y-axis. In each case, the slope of a best-fit line through the origin is given. A dashed line corresponding to a slope of m = 1 is also displayed for reference. The intradomain PREs do not depend on ionic strength, but increasing the salt concentration significantly decreases the magnitude of the interdomain PREs, indicating that the interdomain interactions explored have a significant electrostatic component.

HHS Vulnerability Disclosure