Table 1.

GL15 nucleus morphology analysis during differentiation phases

	Differentiation days
Nucleus typology
	0	3	6	9	12
normal	86 ± 10.2	72 ± 8.3	91 ± 7.5	87 ± 9.4	94 ± 11.3
mitotic	9 ± 2.3	12 ± 2.1	2 ± 0.8	2 ± 1.5	1 ± 0.9
apoptotic	5 ± 1.5	16 ± 3.1	7 ± 2.6	8 ± 2.3	5 ± 1.9

Data derived from DAPI-stained nuclei (see Materials and Methods) observed using a fluorescence microscope. Each value represents the mean ± s.d. of the percentage of nuclei with different morphology: regular lobate nuclei (normal), two distinct nuclear lobes (mitotic) and enriched pluri-lobate nuclei (apoptotic). During selected differentiation times (0, 3, 6, 9 and 12 days), the percentages were calculated from values derived from five randomly selected fields each containing about 50-60 cells on slices, in duplicate for each sample.