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. 2010 May 18;2010:628153. doi: 10.4061/2010/628153

Figure 1.

Figure 1

Agarose gel 1% of the clone fragment of the gene PPE (1080 bp) in pQE-30 UA expression vector. Lane M1: DNA molecular weight marker 100 bp ladder. Lane1: PCR amplified PPE gene fragment of Mycobacterium avium subsp. paratuberculosis. Lane 2: Released insert of 1080 bp PPE gene fragment after digestion with NheI and EcoRI from recombinant plasmid pQE-30 UA PPE. Lane M1: Presented DNA molecular weight marker Lamda DNA/EcoRI/Hind III digest.