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. 2010 May 18;2010:628153. doi: 10.4061/2010/628153

Figure 2.

Figure 2

Coomassie Brilliant Blue stained 15% SDS-PAGE showing expressed PPE protein. Lane M: Prestained protein molecular weight marker. Lane 1: Purified recombinant 37.1 kDa his tag recombinant PPE protein. Lane 2: Whole cell extract of E. coli M15 harbouring 37.1 kDa fusion PPE-6xhistag protein (IPTG induced). Lane 3: Whole cell extract of E. coli M15 harbouring 37.1 kDa fusion PPE-6xhistag protein (IPTG uninduced). Lane 4: Whole cell extract of E. coli M15.