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Dot blot assay showing sero reactivity of polyclonal sera against PPE 37.1 kDa recombinant protein with purified recombinant PPE protein and Mycobacterium avium subsp. paratuberculosisi strain 316F sonicate. Lane 1: PBS negative control. Lane 2: Recombinant purified 37.1 kDa PPE. Lane 3: M. a. paratuberculosis strain 316F sonicate.
