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. 2011 Nov 16;101(10):2360–2369. doi: 10.1016/j.bpj.2011.10.014

Figure 2.

Figure 2

Flow cytometry. (A) Binding curve of anti-IL-4Rα (M57) to a type II IL-4R expressing BaF3 cell line. Numbers of bound PE per cell were calibrated with fluorescent beads. Isotype control PE-labeled MOPC21 (solid square). (B) Binding of IL-4-A488 to IL-4R expressing BaF3 (inset). (C) Simultaneous binding of IL-4-A488 (solid circles) and displacement of PE-labeled M57 (open circles) measured in the respective color channels. (D) Comparative displacement of 10 nM PE-labeled M57 by nonlabeled IL-4 (open circles) or IL-4-488 (solid circles). (E) Confocal images of HEK293T cells transiently expressing type I IL-4R constructs. Imaging with A488 channel before and after administration of IL-4-A488 proves specific binding of the ligand (arrows).