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Single-cell binding kinetics in HEK293T. (A) Confocal cross sections of a HEK293T cell transiently expressing IL-4Ram266-eYFP successively binding the ligand IL-4-A647 (eYFP and A647 channels in gray scale). (B) Time course of the ratio of mean intensities obtained from the images in the area a1 and a2. Administration of ligand IL-4-A647 and competition wild-type IL-4 at indicated concentrations and time points.
