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. 2011 Nov 16;101(10):2426–2435. doi: 10.1016/j.bpj.2011.10.005

Figure 2.

Figure 2

Effect of Chol on the lytic activities and binding capacities of MPER peptides. (A, left) Effect of Chol on CpreTM-induced leakage kinetics. The peptide was added at the time indicated by the arrow (t = 50 s) at a peptide/lipid ratio of 1:500. Lipid concentration was 100 μM. Chol mole fractions are indicated for each curve. (A, right) Partitioning curves estimated from the fractional change in Trp fluorescence with increasing POPC or POPC/Chol (1:1) concentrations (squares and circles, respectively). Peptide concentration was 0.5 μM. Lines correspond to the best fit of the experimental values to a hyperbolic function (see Materials and Methods in the Supporting Material). (B) MPERp peptide was used at a peptide/lipid ratio of 1:150 in the leakage assays (left). Otherwise, conditions are as in A.