Figure 5.

Dose dependency and mechanism of CpreTM-induced content leakage from POPC (circles and solid lines), POPC/Chol (4:1) (squares and dotted lines), and POPC/Chol (2:1) (triangles and dashed lines) LUVs. (A) Partitioning curves estimated from the fractional change in Trp fluorescence with increasing lipid concentration. The fitting curves are superimposed. (B) ANTS leakage (extent measured 60 min after peptide addition) measured as a function of the peptide/lipid mole ratio. The lines link the experimental values for better appreciation of the differences. (C) Fluorescence requenching analysis to establish the mechanism of membrane permeabilization. Internal quenching (Q_in) was measured as a function of the ANTS released (f_out) after incubation with peptide (see Supporting Material).