Table II.

Thermal Denaturation and Aggregation Midpoints

	Thermal denaturation midpoint (°C)	ThioT midpoint (h)	SDS-stability midpoint (h)
Josephin	51.3 ± 0.6	79.4 ± 6.7
Josephin R103G	55.9 ± 0.2	147.5 ± 7.3
Josephin S81A	54.1 ± 0.4	115.2 ± 6.9
Josephin R103G S81A	55.4 ± 0.2	>150 h
Josephin L169H	47.5 ± 0.1	4.0 ± 0.9
Ataxin-3(Q15)	49.2 ± 0.1	24.0 ± 1.3
Ataxin-3(Q15) R103G	52.9 ± 0.8	48.9 ± 1.8
Ataxin-3(Q15) S81A	50.6 ± 0.9	32.2 ± 1.9
Ataxin-3(Q15) R103G S81A	53.4 ± 0.4	50.0 ± 2.8
Ataxin-3(Q64)	51.0 ± 0.3	11.7 ± 1.2	35.0 ± 2.7
Ataxin-3(Q64 R103G	52.1 ± 0.6	25.3 ± 2.1	42.9 ± 2.6
Ataxin-3(Q64) S81A	50.8 ± 0.2	26.3 ± 4.9	41.0 ± 7.1
Ataxin-3(Q64) R103G S81A	52.4 ± 0.3	40.6 ± 2.2	48.7 ± 1.3