Table III.
Interpeptide and Intrapeptide Crosslinks for αB-crystallin
| Theoretical [M+H]+ | Matched sequence(s) and modification | Modified amino acid residues | Distance Ca–Ca (Å) | Mono-, di-, or oligomer | ||
|---|---|---|---|---|---|---|
| 2222.07 | * | M1DIAIHHPWIR11 + L70EKDR74 | 1 | 72 | — | unstr. |
| 2954.52 | * | M1DIAIHHPWIR11 + V91KVLGDVIEVHGK103 | 1 | 92 | — | unstr. |
| 2027.98 | * | M1DIAIHHPWIR11 +K121YR123 | 1 | 121 | — | unstr. |
| 2462.19 | * | M1DIAIHHPWIR11+ K150QVSGPER157 | 1 | 150 | — | unstr. |
| 2830.42 | M1DIAIHHPWIR11 + E164EKPAVTAAPKK175 | 1 | 166/174/175 | — | unstr. | |
| 1493.71 | * | L70EKDR74 + L70EKDR74 | 72 | 72 | — | o |
| 2047.00 | L70EKDR74 + H83FSPEELKVK92 | 72 | 90 | — | o | |
| 1299.62 | * | L70EKDR74 + K121YR123 | 72 | 121 | 24 | m |
| 1733.83 | * | L70EKDR74 + K150QVSGPER157 | 72 | 150 | 10 | m |
| 2102.06 | L70EKDR74 + E164EKPAVTAAPKK175 | 72 | 166/174/175 | — | m | |
| 2294.05 | * | L70EKDR74 + E164EKPAVTAAPKK175 Hydr | 72 | 166/174/175 | — | m |
| 1973.97 | * | L70EKDR74 + E164EKPAVTAAPK174 | 72 | 166 | — | m |
| 3103.53 | * | H83FSPEELKVK92 + V93LGDVIEVHGKHEER107 | 90 | 103 | 9 | m |
| 2118.05 | * | V91KVLGDVIEVHGKHEER107 Intra | 92 | 103 | 14 | m |
| 2032.07 | * | V91KVLGDVIEVHGK103 + K121YR123 | 92 | 121 | 10 | m |
| 2466.28 | * | V91KVLGDVIEVHGK103 + K150QVSGPER157 | 92 | 150 | 22 | m |
| 2356.15 | * | V93LGDVIEVHGKHEER107 + K121YR123 | 103 | 121 | 16 | m |
| 1539.74 | * | K121YR123 +K150QVSGPER157 | 121 | 150 | 22 | m |
| 1779.88 | * | K121YR123 +E164EKPAVTAAPK174 | 121 | 166 | — | m |
| 1973.95 | * | K150QVSGPER157 + K150QVSGPER157 | 150 | 150 | — | o |
| 2342.18 | * | K150QVSGPER157 + E164EKPAVTAAPKK175 | 150 | 166/174/175 | — | m |
| 2534.18 | * | K150QVSGPER157 + E164EKPAVTAAPKK175 Hydr | 150 | 166/174/175 | — | m |
| 2214.09 | * | K150QVSGPER157 + E164EKPAVTAAPK174 | 150 | 166 | — | m |
| 1442.70 | * | E164EKPAVTAAPKK175 Intra | 166/174 | 174/175 | — | m |
Crosslinks indicated with an asterisk (*) were also detected in a crosslinking experiment using BS3. Intra, intrapeptide crosslink; Hydr, hydrolyzed dead-end crosslinker modification; m, monomers; d, dimers; o, oligomers; unstr., unstructured regions.