Figure 2.

Identification of mouse DNA in human pancreatic carcinoma cultures established from mouse xenografts by PCR. A sensitive PCR assay specific for mouse GAP DH and its pseudogenes was used (Fig. S2 and Materials and Methods). The genomic DNA of human pancreatic carcinoma XCL1 and XCL3 xenograft cultures were found positive for mouse DNA. By contrast, the DNA of human pancreatic carcinoma CAK1 and human colorectal carcinoma RKO culture as well as the control pooled human WBC DNA were free mouse DNA (see Materials/Methods and Table S1–3).