Table 3.
Frequent detection of murine leukemia virus (MLV) contamination of non-xenograft human cultures
| Lab PI | Non-xenograft cell line type | No. of MLV postive cell lines1/No. of total cell lines tested (%) |
| Xenograft culture lab | ||
| A. Gazdar (NCI)2 | NSCLC | 1/16 (5%)* |
| A. Gazdar (NCI)2 | SCLC | 5/37 (13%)* |
| C. Rudin | NSCLC | 1/4 (25%) |
| C. Rudin | SCLC | 2/4 (50%) |
| A. Maitra3 | Pancreas | 0/9 (0%) |
| A. Maitra | Colon | 1/1 (100%) |
| R. Brekken3 | Pancreas | 0/2 (0%) |
| J.T. Hsieh | Prostate | 3/7 (43%) |
| Total: 13/78 (17%) | ||
| Xenograft culture free lab | ||
| A. Gazdar (UTSW)4 | NSCLC | 0/33 (0%)* |
| A. Gazdar (UTSW)4 | SCLC | 0/17 (0%)* |
| Total: 0/50 (0%) |
1
The cell lines were strongly positive by two or three viral probes by MLV qPCR and confirmed by the partial sequences of gag and env regions or full genome sequence (see Materials/Methods for the details and Table 4). See Table S2 for the list of cell lines tested.
2
A. Gazdar's former lab at NCI.
3
The DNA of two cell lines (MIAP aCa-2 and PA NC-1) from both labs was tested.
4
A. Gazdar's current lab at UTSW.
*
p = 0.01, Fisher exact, 2-tailed test.