FIG. 5.

Sub-acute permeability alterations. Representative confocal reconstructions of 3-D neuronal-astrocytic co-cultures following mechanical loading at 0.50 shear strain, 10 sec⁻¹ strain rate. Calcein was added to the cultures either prior to loading (0⁻, A), or 10 min post-loading (B). There was modest uptake when the calcein was present during loading, but minimal uptake when calcein was added minutes post-insult (reconstructions from 50-μm-thick z-stacks imaged post-rinse).