FIG. 9.

Regression analyses to assess potential correlations between acute markers of cell damage and subsequent longer-term cell death. Cell death versus the density of permeabilized cells (A) or versus the degree of per-cell permeability (B). The degree of calcein uptake on a per cell basis (B) was a stronger indicator of cell death than the density (or percentage) of calcein⁺ cells (A), implicating the degree of damage rather than the presence of damage in subsequent cell death. Additionally, this increase in cell death was markedly greater for shear versus compression across all strain rates, substantiating that shear deformation is the prevalent mechanism of cellular damage that ultimately induces cell death. Data: mean±SEM.