Figure 3.

PI3K signaling is activated by sfRon and is required for the invasive activity of sfRon. (A) Western blot on whole-cell lysates from parental MCF7 cells or those expressing Ron or sfRon, using antibodies specific for Akt or Erk (phosphorylated or total). (B) Addition of PI3K inhibitors Ly294002 (40 uM) or wortmannin (1, 5, or 10 nM) blocked sfRon-induced invasion. Error bars reflect standard deviation from at least 3 experimental replicates. (C) Western analysis of N-cadherin on wortmannin-treated MCF7-sfRon cells. The results revealed that blocking PI3K signaling could not reverse sfRon-induced EMT.