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. 2011 Jul;2(7):720–727. doi: 10.1177/1947601911425832

Figure 2.

Figure 2.

Cisplatin treatment in miR-203 knockdown MCF-7 cells induces apoptotic cell death. (A) MCF-7 cells were transduced with replication-deficient lentivirus expressing anti-miR-203 or control miRNA, and stable cell lines were generated. Cells were then treated with cisplatin at different time points, and cell viability was determined by trypan blue exclusion. Results are presented as the mean of three separate experiments with standard errors (**P < 0.001). (B) MCF-anti-miR-203 and MCF-Cont (anti-miR) cells were treated with cisplatin for 48 hours, and cell lysates were subjected to Western blot analysis using specific antibody for detection of caspase-9, caspase-7, or poly(ADP-ribose) polymerase (PARP). Caspase-9 and caspase-7 activation was observed in cisplatin-treated MCF-anti-miR-203 cells. PARP was significantly cleaved to an 86-kD signature peptide (cPARP) in cisplatin-treated MCF-anti-miR-203 cells as compared to MCF-Cont. The blot was reprobed with an antibody to actin for comparison of equal protein load.