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. 2011 Jul;2(7):720–727. doi: 10.1177/1947601911425832

Figure 5.

Figure 5.

Knockdown of miR-203 enhances suppressor of cytokine signaling 3 (SOCS3) expression in MCF-7 cells. (A) MCF-Cont and MCF-anti-miR-203 or MDA-231-Cont and MDA-231-anti-miR-203 cell lysates were analyzed for SOCS3 protein expression by Western blot. The blot was reprobed with actin antibody to compare protein load. (B) MCF-anti-miR-203 and MCF-Cont cell treated with cisplatin displayed a significantly higher level of SOCS3 expression. The blot was reprobed with an antibody to actin for comparison of equal protein load. (C) SOCS3 sensitizes MCF-7 cells to cisplatin. MCF-Cont and MCF-7 SOCS3 cells were treated with 50 µM cisplatin for 24 hours. Cell lysates was prepared and probed with poly(ADP-ribose) polymerase (PARP) antibody. The blot was reprobed with an antibody to actin for comparison of equal protein load. cPARP = cleaved PARP.