Skip to main content
. Author manuscript; available in PMC: 2011 Nov 17.
Published in final edited form as: Cancer Biol Ther. 2008 Oct 3;7(10):1584–1590. doi: 10.4161/cbt.7.10.6562

Figure 3.

Figure 3

Nanoparticle-DT-A DNA delivery to MSLN+ cells inhibits protein synthesis dramatically and affects overall cell survival. Luciferase activity measured after (A) 24 hour and (B) 48 hour post-delivering MSLN+ cell lines, Hs766T and CAPAN1, with MSLN/CAG-Luc DNA and MSLN/DT-A/CAG-luc DNA. Experiments were performed in triplicate (error bars represent SD, RLU = relative light units). (C) Cell survival assays of MSLN+ pancreatic cancer cells, Hs766T, and the MSLN− pancreatic cancer cell line, PL5. Total number of viable cells was enumerated manually 6 days post-delivery by trypan blue staining. Percent viability was determined by calculating total number of viable cells compared to untreated cultures. Experiments were performed in duplicate with two counts made for each well (Error bars represent SEM). We further determined that 34% of the total cells counted (viable plus dead) for the MSLN+ line on day 6 were dead, as assayed by trypan blue exclusion.