Figure 3A.

The effects of ANOE on the heart contractile activity in Ca-depleted medium.

The control (Ca 100%) represents the contractile force (CF) and heart rate (HR) of the heart recorded in normal Mac-Ewen physiological solution. In this experiment ANOE was used in the single concentration of 0.1µg/ml. Ordinate: CF is expressed in millimetres, while HR is given as the number of contractions/minute. Abscissa: super-fusion media. Data are expressed as means ± SEM (n = 15). In calcium-depleted medium, for CF: **, P < 0.01 for Ca 75% + ANOE vs. control; and #, p < 0.05 for Ca 75% vs Ca 5% + ANOE. For HR: **, P < 0.01 for Ca 75% vs control, ***, P < 0.001 for Ca 75% + ANOE vs. control, and ###,?? P < 0.001 for Ca 75% vs Ca 75% + ANOE. In calcium-enriched medium, for HR: ***, P < 0.01 for Ca 125% and Ca 125% + ANOE vs control, and ###??, P < 0.001 for Ca 125% vs Ca 125% + ANOE.